For a list of HTRF-compatible readers and set-up recommendations, please visit . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. For more information about HTRF. VICTOR Nivo Multimode Plate Reader. e. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. When MikroWin opens, if you already have a pre-existing template for. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The optional HTRF-certified filter-based module may be. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Unlike standard ELISA plate readers, MSD. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. For a list of HTRF-compatible readers and set -up recommendations, please visit. Lyse cells Read on an HTRF compatible reader . + 4 µL HTRF ® pre-mixed antibodies . SV detection plate. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information about HTRF. For more ® information about HTRF compatible readers and for set-up recommendations, please. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. HTRF is compatible with a large range of available microplate readers. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Incubate overnight at RT. PURCHASE SEPARATELY . The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The kit includes everything you need: reagents, buffers and a microplate. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. VICTOR® Nivo™ multimode plate reader 2 | For research use only. After an overnight incubation, the plate is washed 3 times. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Make sure the setup for Tb Cryptate is used. Its. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. For instance, when assay volumes are. Make sure the setup for Eu Cryptate is used. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. It accepts all types of microtitration plate. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 96-well or 384-well small volume (SV) detection. For more information about HTRF ®Seal the plate and incubate for Overnight. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. 10 µL Standard or Sample. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your. For more. For more information about HTRF. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). HTRF-compatible SpectraMax Multi-Mode Readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. . FOR HTRF CERTIFIED. Handle complex biological matrices easily. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. With its ease of use software and remote access from almost any device, it is ideal for multiuser environments. This protocol, HTS designed, allows miniaturization while. Microplate Readers and Reader Accessories. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. Make sure the setup for Eu3+ Cryptate is used. The plate was covered and incubated for one hour at room temperature. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Specialized filters allow for IR fluorescence. Seal the plate and. FOR HTRF CERTIFIED READER. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. protocol with suspension cells or with a. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. For more information about HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. VICTOR® Nivo™ multimode plate reader. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. This protocol gives the cells viability and confluence to be monitored. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It measures at any wavelength, with top and bottom reading for most standard technologies. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. For more information. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Incubate 4h at RT. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. FOR HTRF CERTIFIED READER. HTRF is compatible with a large range of available microplate readers. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Even if not listed, some. FlexStation® 3 Multi-Mode Microplate Reader. Your lab might even have one already. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. FOR HTRF CERTIFIED READER. 1) . Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. Figure 1. HTRF Microplate Readers. 96-well culture plateHTRF certified . The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. FOR HTRF CERTIFIED READER For. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plus . FOR HTRF CERTIFIED READER For more information about HTRF. pre+ 5 µL HTRFcompounds Activation Buffer. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. Incubate overnight at RT. At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. For the standard curve described, all wells of the microplate are read. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. $352. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . 0. Remove the plate seal and read on an HTRF compatible reader. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. (665 nm and 620 nm) on a compatible HTRF ® reader. For more information. Homogeneous Time-Resolved Fluorescence (HTRF) . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more information about HTRF ® compatible readers and for set-up recommendations, please. FOR HTRF CERTIFIED READER. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. To perform the assay, use white plate only. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. 3 and higher include optimized reader settings, as well as data analysis, for faster results. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. FOR HTRF CERTIFIED READER. Read & analyse. The lysis buffer 4X is ready-to-use. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. No washing steps are required. and Canaa -8-963-4567. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Simply place the module into one of the five module bays and read the assay. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Read & analyse. FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. MATERIALS PROVIDED: KIT . For instance, when assay volumes are. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. EGFR protein (PV3872, 0. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. 3-in-1 absorbance using a variety of sample formats, including microplates. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. FOR HTRF CERTIFIED READER For more information about HTRF. COMPONENTS ; STORAGE . Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The CLARIOstar® Plus is our most flexible multi-mode plate reader. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. The optical systems use two scanningHighly compatible plates for HTRF. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. After defining the objectives and their locations in Gen5: 1. 1. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. If not, our scientific team will help you choose the instrument that best suits your needs and budget. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. Customer Transformation Hub. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. This protocol, HTS designed, allows miniaturization while. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. At Cisbio we provide microplates that help you discover better. Microplate readers are only certified if they. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. HTRF-specific. PerkinElmer. 5 nm filter (PerkinElmer 2100-5110) were used. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. HTRF certified . The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. The VICTOR Nivo is a HTRF certified microplate reader. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. SpectraMax i3/i3x and Paradigm readers can be equipped with. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. use of a wide variety of plates or sample matrix formats. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Cover the plate with a plate sealer. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. Quantify up to 10 analytes per well. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Remove the plate sealer and read on an HTRF® compatible reader . Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. Make sure the setup for Tb Cryptate is used. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. FOR HTRF CERTIFIED READER. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. 15 / Each of 1; Qty. For more ® information about HTRF compatible readers and for set-up recommendations, please. These plates can be read on all HTRF certified readers, thus making their implementation in. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. Conclusion. Incubate overnight at RT. This protocol, HTS designed, allows. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Incubate 4h at RT. Make sure the setup for Tb Cryptate is used. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Introduction. • HTRF®-Certified Reader **. PerkinElmer. On the SpectraMax i3x reader, install the HTRF cartridge, and wavelengths will be selected automatically when HTRF is selected as the Optical Configuration. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. " The PHERAstar FSX instrument is a high quality, reliable, plate reader. Taking these considerations into account, Cisbio’s 96w low volume and PKI’s 96w & 384w microplates have been carefully designed to provide the best signal detection environment possible to all HTRF assays. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. Plate 25 μL of cells in. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. • HTRF®-Certified Reader. * For HTRF microplate recommendations, please visit . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. (donor) of compounds are measured on an HTRF-compatible reader. Read & analyse. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. overnight at 4 C [Prostaglandin E2] pg/ml. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. HTRF-specific. 5. Read & analyse. Make sure the setup for Eu3+ Cryptate is used. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Due to the chemical and photophysical. Highly compatible plates for HTRF. . The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. Revvity. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. There are multiple factors to consider including: detection type, additional. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. S. Revvity. 1) . For a list of HTRF-compatible readers and set-up recommendations, please visit . FOR HTRF CERTIFIED. . Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. 27 / Pack of 100; Qty. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. It enables you to gain insights you couldn’t achieve with detection measurements alone. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. The ratio between control lysate signal / non-specific signal should be greater than 2. acceptor reagent. Read & analyse. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. For more. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. High Speed Monochromator offers a typical wavelength accuracy of <0. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. This protocol gives the cells viability and confluence to be monitored. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. 5% and an increased Z’ value of 0. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. In addition, stacker. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. At Cisbio we provide microplates that help you discover better. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. The assay was prepared in both white and black plates and incubated >18 hours. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER For more. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. NanoQuant Plate™ allows rapid, straightforward measurement of 16 x 2 µl samples simultaneously without calibration. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. • HTRF®-Certified Reader. FOR HTRF CERTIFIED READER. Cell seeding densities of 2,000,000 cells/well are generally sufficient for most cell lines, but. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. PURCHASE SEPARATELY . This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For a list of HTRF-compatible readers and set-up recommendations, please visit . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Eu cryptate emission level). Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Make sure the setup for Eu3+ Cryptate is used. We would like to show you a description here but the site won’t allow us. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. If not, our scientific team will help you choose the instrument that best suits your needs and budget. For more information about HTRF. For more information. EX: HTRF Emission range. This protocol gives the cells viability and confluence to be monitored. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Conclusion. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. COMPONENTS ; STORAGE . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. Its. These plates can be read on all HTRF certified readers, thus making their implementation in. Lyse cells Read on an HTRF compatible reader . 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. + 4 µL HTRF ® pre-mixed antibo dies 2. FLUOstar Omega Filter-based multi-mode microplate reader . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. 3. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Figure 1: Principle of HTRF Cortisol competitive assay. HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. Read & analyse. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER For more information about HTRF.